Active surveillance of adult healthcareassociated infections in intensive care units: resistance and molecular profile in an upper middle-income country

Objective: This study aimed to characterize epidemiological and molecular profile of Healthcare-associated infections [HAI] in 21 intensive care units (ICU) a city Colombia. Methods: Descriptive prevalence. Adult patients were screened ICUs for HAIs: VAP, CLABSI; CAUTI and/or SSI. Microbiological genotypic identification was performed. Results: Prevalence HAIs 41.4% (CI 36.9-45.9).VAP 15.8% 12.7-19.4); CLABSI, 13.5% 10.6-16.9); CAUTI, 7.7% 5.5-10.5); SSI, 4.4% 2.7-6.6). Gram-negative bacteria (71.7%) predominated (P. aeruginosa (19.1%), K. pneumoniae (13.4%) E. coli (13%)). Pseudomonas spp. 20-30% resistant carbapenems greater than 10% aztreonam, 3rd- 4th-generation cephalosporins, Beta-lactamase inhibitors. In VAP 30% Staphylococcus aureus oxacillin. epidermidis exhibited 100% resistance. P. resistance gene blaTEM, blaSHV, blaCTX-M (15- 32%), KPC (5.7%), oxacillinases blaOXA-48 (1.8%) blaOXA-1-40-30 (20-50%). coli, genes qnrB, qnrS qnrD identified. ermC-type (16.7%), aph[2’]’If (7.7%) ant[4’]-Ia identified aureus. Conclusions: CLABSI predominate evaluated Colombia due gram-negative by ESBL-type plasmids, efflux pumps hindering the therapeutic approach.